bsm-33221M [Primary Antibody]
GRP78/Bip Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: GRP78/Bip

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3309

Swiss Prot: P11021

Source: KLH conjugated synthetic peptide derived from human GRP78/Bip

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 78


Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jianzhao Liao. et al. Endoplasmic Reticulum Stress Contributes to Copper-Induced Pyroptosis via Regulating the IRE1_-XBP1 Pathway in Pig Jejunal Epithelial Cells. J Agr Food Chem. 2022;70(4):12931303Read more>>
VALIDATION IMAGES

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GRP78/Bip) monoclonol Antibody, Unconjugated (bsm-33221M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.