bsm-33223M [Primary Antibody]
STAT3 (5H4) Monoclonal antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: STAT3

Specificity: KO-Validated

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6774

Swiss Prot: P40763

Source: Recombinant human STAT3 Protein

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. Gene Symbol: APRF; FLJ20882; HIES; MGC16063.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Predicted Molecular Weight: 88kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Feng\Feng Lv. et al. microRNA\495 alleviates endothelial cell inflammatory injury in coronary heart disease via blockade of the NF\B pathway by targeting RALB. 2020 Aug 18Read more>>
  • Keliang Song. et al. Knocking down Siglec-15 in osteosarcoma cells inhibits proliferation while promoting apoptosis and pyroptosis by targeting the Siglec-15/STAT3/Bcl-2 pathway. ADV MED SCI-POLAND. Adv Med Sci-Poland. 2022 Sep;67:187Read more>>
  • Taojie Zhang. et al. MEL regulates miR-21 and let-7b through the STAT3 cascade in the follicular granulosa cells of Tibetan sheep. THERIOGENOLOGY. 2023 ApRead more>>
  • Liqun Wang. et al. Global profiling of miRNA and protein expression patterns in rabbit peritoneal macrophages treated with exosomes derived from Taenia pisiformis cysticercus. GENOMICS. 2023 Sep;115:11069Read more>>
VALIDATION IMAGES

Lane 1: Mouse Heart lysates; Lane 2: Mouse Placenta lysates; Lane 3: Mouse Lung lysates probed with STAT3 Polyclonal Antibody, Unconjugated (bsm-33223M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (5H4) Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (5H4) Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:200 overnight at 4°C, DAB staining.


Lane 1: STAT3 knockout (KO) A549 cell lysates; Lane 2: Human A549 cell (Control) lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human SW480 cell lysates; Lane 6: Human THP-1 cell lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.