bsm-33236M [Primary Antibody]
AMPK alpha-1 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: AMPK alpha-1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5562

Swiss Prot: Q13131

Source: KLH conjugated synthetic peptide derived from human AMPK alpha-1

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 64 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yanli Guo. et al. Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucoseCtreated HK-2 cells. Biomed Pharmacother. 2020 Nov;131:110684Read more>>
  • Jinjing Jia. et al. Thioredoxin-1 Promotes Mitochondrial Biogenesis Through Regulating AMPK/Sirt1/PGC1 Pathway in Alzheimer's Disease. ASN NEURO. ;()Read more>>
  • Danning Tong. et al. Aspirin alleviates cisplatin-induced acute kidney injury through the AMPK-PGC-1 signaling pathway. CHEM-BIOL INTERACT. 2023 Aug;380:110536Read more>>
VALIDATION IMAGES

HL60 cell lysates probed with AMPK alpha-1 Polyclonal Antibody, Unconjugated (bsm-33236M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.