bsm-33278M
[Primary Antibody]
AKT1 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: AKT1
Immunogen Range: 420-479/479
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 207
Swiss Prot: P31749
Source: KLH conjugated synthetic peptide derived from human AKT1
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Size: 100ul
Concentration: 1ug/ul
Applications: WB(1:300-5000)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- X Zhou et al. Modulating NMDA receptors to treat MK-801-induced schizophrenic cognition deficit: effects of clozapine combining with PQQ treatment and possible mechanisms of action. BMC Psychiatry. 2020 Mar 6;20(1):106.Read more>>
- Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci. 2020 Oct 1;258:118147.Read more>>
- Le Tian. et al. Luteolin as an adjuvant effectively enhances CTL anti-tumor response in B16F10 mouse model. Int Immunopharmacol. 2021 May;94:107441Read more>>
- Yilei Li. et al. Vitellogenin 2 promotes muscle development and stimulates the browning of white fat. Aging-Us. 2021 Oct 15; 13(19): 22985C23003Read more>>
- Chen Luyao. et al. Epicatechin gallate prevents the de novo synthesis of fatty acid and the migration of prostate cancer cells. Acta Bioch Bioph Sin. 2021 OcRead more>>
- Chen, Yao. et al. The potential effects and mechanisms of_hispidulin in the treatment of diabetic retinopathy based on_network pharmacology. BMC Complementary Medicine and Therapies. 2022 Dec;22(1):1-12Read more>>
- Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635Read more>>
- Wang, Weili. et al. Engineering micro oxygen factories to slow tumour progression via hyperoxic microenvironments. NAT COMMUN. 2022 Aug;13(1):1-17Read more>>
- Xiao-Jiao Chen. et al. Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways. MOLECULES. 2022 Jan;27(19):6435Read more>>
- Rui Sun. et al. Construction of crizotinib resistant models with CD74-ROS1 D2033N and CD74-ROS1 S1986F point mutations to explore resistance mechanism and treatment strategy. CELL SIGNAL. 2023 Jan;101:110497Read more>>
- Yang, Chong. et al. TRIM15 forms a regulatory loop with the AKT/FOXO1 axis and LASP1 to modulate the sensitivity of HCC cells to TKIs. CELL DEATH DIS. 2023 Jan;14(1):1-13Read more>>
- Ying Gao. et al. ZYY-B-2, a novel ALK inhibitor, overcomes resistance to ceritinib by inhibiting P-gp function and induces apoptosis through mitochondrial pathway in ceritinib-resistant H2228?cells. CHEM-BIOL INTERACT. 2023 Jul;379:110516Read more>>
- Li Li. et al. Regulation of TREM2 on BV2 inflammation through PI3K/AKT/mTOR pathway. BIOTECHNOL GENET ENG. 2023 Apr 26Read more>>
- Li Gu. et al. Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex. PHARMACOL RES. 2023 Jun;192:106789Read more>>
- Qianfeng Liu. et al. Perfluoroalkyl substances promote breast cancer progression via ER and GPER mediated PI3K/Akt and MAPK/Erk signaling pathways. ECOTOX ENVIRON SAFE. 2023 Jun;258:11498Read more>>
- Junfeng Wang. et al. Overexpression of TYRO3 indicates poor prognosis and induces gastric cancer progression via AKT-mTOR pathway. MOL CARCINOGEN. 2023 MayRead more>>
- Zhao Jiamin. et al. Sea buckthorn oil regulates primary myoblasts proliferation and differentiation in vitro. IN VITRO CELL DEV-AN. 2023 Dec;:1-12Read more>>
VALIDATION IMAGES
Lane 1: MCF-7 cell lysates; Lane 2: A549 cell lysates; Lane 3: Hela cell lysates probed with AKT Monoclonal Antibody, Unconjugated (bsm-33278M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.