bsm-33284M
[Primary Antibody]
Caspase-3 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: Caspase-3
Immunogen Range: 1-100/277
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 836
Swiss Prot: P42574
Source: KLH conjugated synthetic peptide derived from human Caspase-3
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Reacts with Caspase-3 subunit p17 and precursor.
Reacts with Caspase-3 subunit p17 and precursor.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)
Predicted Molecular Weight: 17/28 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Gao Y et al. Effect of splenectomy on attenuation of LPS-induced AKI through GTS-21-induced cholinergic anti-inflammatory pathway. Am J Transl Res. 2019 Apr 15;11(4):2540-2549. Read more>>
- Shuli Shao. et al. Toosendanin induces apoptosis of MKN?45 human gastric cancer cells partly through miR?23a?3p?mediated downregulation of BCL2. Mol Med Rep. 2020 Sep;22(3):1793-1802Read more>>
- Yang Gao. et al. Mechanisms of Renal-Splenic Axis Involvement in Acute Kidney Injury Mediated by the 7nAChR-NF-B Signaling Pathway. 2020 Nov 03Read more>>
- Deng Zhiqin. et al. Interleukin 1 betaCinduced chloride currents are important in osteoarthritis onset: an in vitro study. Acta Bioch Bioph Sin. 2021 MaRead more>>
- Shunshun Han. et al. Fibromodulin is involved in autophagy and apoptosis of granulosa cells affecting the follicular atresia in chicken. Poultry Sci. 2021 Oct;:101524Read more>>
- Keliang Song. et al. Knocking down Siglec-15 in osteosarcoma cells inhibits proliferation while promoting apoptosis and pyroptosis by targeting the Siglec-15/STAT3/Bcl-2 pathway. ADV MED SCI-POLAND. Adv Med Sci-Poland. 2022 Sep;67:187Read more>>
- Jieqiong Zhang. et al. Irisin Attenuates Pathological Neovascularization in Oxygen-Induced Retinopathy Mice. INVEST OPHTH VIS SCI. 2022 Jun;63(6):21-21Read more>>
- Danyu Wang. et al. Photoactivated DNA Nanodrugs Damage Mitochondria to Improve Gene Therapy for Reversing Chemoresistance. ACS NANO. 2023;XXXX(XXX):XXX-XXXRead more>>
- Lisha Peng. et al. miR-128-3p increases the radiosensitivity in nasopharyngeal carcinoma via regulating vascular endothelial growth factor C. PATHOL RES PRACT. 2023 Aug;:154768Read more>>
- Liu Xueqi. et al. In Vivo Evaluation of Self-assembled nano-Saikosaponin-a for Epilepsy Treatment. MOL BIOTECHNOL. 2023 Aug;:1-11Read more>>
- Wenting Li. et al. A collective hyperthermia-facilitated glutathione inhibition strategy for boosted oxidative stress treatment. CHEM ENG J. 2024 Apr;485:150056Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (bsm-33284M) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (bsm-33284M) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (bsm-33284M) at 1:400 overnight at 4°C, DAB staining.