bsm-33293M
[Primary Antibody]
GSK3B (3A6) Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: GSK3B
Specificity: KO-Validated
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 2932
Swiss Prot: P49841
Source: KLH conjugated synthetic peptide derived from human GSK-3 Beta
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
Dog
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang Z et al. Interaction between Endothelin-1 and Left Stellate Ganglion Activation: A Potential Mechanism of Malignant Ventricular Arrhythmia during Myocardial Ischemia. Oxid Med Cell Longev. 2019 May 12;2019:6508328. Read more>>
- Junying Lan. et al. Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model. Neuropharmacology. 2021 Sep;196:108689Read more>>
- Wang, Lili. et al. Progranulin improves neural development via the PI3K/Akt/GSK-3_ pathway in the cerebellum of a VPA-induced rat model of ASD. Transl Psychiat. 2022 Mar;12(1):1-14Read more>>
- Zhao-Yu Zhang. et al. Knockdown of CD146 promotes endothelial-to-mesenchymal transition via Wnt/-catenin pathway. PLOS ONE. 2022 Aug;17(8):e0273542Read more>>
VALIDATION IMAGES
293T lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
A549 lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
K562 lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GSK3B (3A6) Monoclonal Antibody, Unconjugated (bsm-33293M) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: HeLa (30ug), Lane 2: HeLa KO GSK-3 Beta cell Lysate (30ug) probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GSK3B (3A6) Monoclonal Antibody, Unconjugated (bsm-33293M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GSK3B (3A6) Monoclonal Antibody, Unconjugated (bsm-33293M) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Pancreas lysates; Lane 3: Rat Muscle lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Human Hela cell lysates; Lane 6: Human MCF-7 cell lysates; Lane 7: Human A431 cell lysates; Lane 8: Human U-2OS cell lysates probed with GSK3B Monoclonal Antibody, Unconjugated (bsm-33293M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.