bsm-33294M [Primary Antibody]
GSK-3 Beta Monoclonal Antibody
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DATASHEET

Host: Mouse

Target Protein: GSK-3 Beta

Immunogen Range: 1-100/420


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: P49841

Swiss Prot: 2932

Source: KLH conjugated synthetic peptide derived from human GSK-3 Beta

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:500-2000)
IHC-P(1:200-800)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GSK-3 Beta Monoclonal Antibody, Unconjugated (bsm-33294M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSK-3 Beta Monoclonal Antibody, Unconjugated (ascites of bsm-33294M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with GSK-3 Beta monoclonal antibody, unconjugated (bsm-33294M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSK-3 Beta Monoclonal Antibody, Unconjugated (bsm-33294M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSK-3 Beta Monoclonal Antibody, Unconjugated (bsm-33294M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSK-3 Beta Monoclonal Antibody, Unconjugated (bsm-33294M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSK-3 Beta Monoclonal Antibody, Unconjugated (bsm-33294M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSK-3 Beta Monoclonal Antibody, Unconjugated (bsm-33294M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSK-3 Beta Monoclonal Antibody, Unconjugated (bsm-33294M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.