VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Rat Cerebrum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Human U251 cell lysates; Lane 4: Human Siha cell lysates; Lane 5: Rat Cerebellum lysates; Lane 6: Mouse Cerebellum lysates; Lane 7: Rat Testis lysates; Lane 8: Mouse Testis lysates probed with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Rat Cerebellum tissue lysates probed with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.