Host: Mouse
Target Protein: Gamma Tubulin
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 7283
Swiss Prot: P23258
Source: KLH conjugated synthetic peptide derived from human Gamma Tubulin
Purification: Purified by Protein G.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.
The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.
Conjugation: ALEXA FLUOR® 750
Excitation/ Emission: 749nm/775nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 50 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.