bsm-33409M
[Primary Antibody]
Collagen II(9H12) Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: Collagen II
Immunogen Range: 1301-1400/1487
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 1280
Swiss Prot: P02458
Source: KLH conjugated synthetic peptide derived from human Collagen II
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Size: 100ul
Concentration: 1ug/ul
Applications: IHC-P(1:200-400)
Predicted Molecular Weight: 142 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Xuejun Dai. et al. Comparison of the differentiation abilities of bone marrow?derived mesenchymal stem cells and adipose?derived mesenchymal stem cells toward nucleus pulposus?like cells in three?dimensional culture. Exp Ther Med. 2021 Sep;22(3):1-9Read more>>
- Chu, Wenhui. et al. The use of a novel deer antler decellularized cartilage-derived matrix scaffold for repair of osteochondral defects. J Biol Eng. 2021 Dec;15(1):1-12Read more>>
- Qianzhi Zhuang. et al. Expressions of parathyroid hormone-related protein (PTHrP) and parathyroid hormone receptor-1 (PTH1R) in the condylar cartilage of temporomandibular joint modulated by occlusal elevation. J DENT SCI. 2022 AugRead more>>
- Yiyun Zhang. et al. Locally delivered modified citrus pectin - a galectin-3 inhibitor shows expected anti-inflammatory and unexpected regeneration-promoting effects on repair of articular cartilage defect. BIOMATERIALS. 2022 Dec;291:12187Read more>>
- Zhenyin Chen. et al. Zonally Stratified Decalcified Bone Scaffold with Different Stiffness Modified by Fibrinogen for Osteochondral Regeneration of Knee Joint Defect. ACS BIOMATER-SCI ENG. 2022;XXXX(XXX):XXX-XXXRead more>>
- Jintao Li. et al. Controlled-release hydrogel loaded with magnesium-based nanoflowers synergize immunomodulation and cartilage regeneration in tendon-bone healing. BIOACT MATER. 2024 Jun;36:62Read more>>
- Shanchao Luo. et al. A novel cartilage-targeting MOF-HMME-RGD sonosensitizer combined with sonodynamic therapy to enhance chondrogenesis and cartilage regeneration. FRONT BIOENG BIOTECH. 2024; 12: 133953Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Collagen II(9H12)) Monoclonal Antibody, Unconjugated (bsm-33409M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat fetal bone); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Collagen II(9H12)) Monoclonal Antibody, Unconjugated (bsm-33409M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Collagen II(9H12)) Monoclonal Antibody, Unconjugated (bsm-33409M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Collagen II(9H12)) Monoclonal Antibody, Unconjugated (bsm-33409M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.