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Host: Mouse
Target Protein: Mart-1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 2315
Swiss Prot: Q16655
Source: KLH conjugated synthetic peptide derived from human Mart-1
Purification: Purified by Protein A.
Storage Buffer: PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:500-2000))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))
Predicted Molecular Weight: 13
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mart-1 Monoclonal Antibody, Unconjugated (bsm-34121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with Mart-1 monoclonal antibody, unconjugated (bsm-34121M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.