bsm-41320R [Primary Antibody]
TLR7 Recombinant Antibody
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Host: Rabbit

Target Protein: TLR7

Immunogen Range: 24-348/1049


Clonality: Recombinant

Isotype: IgG

Entrez Gene: 51284

Swiss Prot: Q9NYK1

Source: Recombinant human TLR7 protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(WB(1:500-2000))
FCM(FCM(1ug/Test))

Predicted Molecular Weight: 121


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Recombinant human TLR7 protein, His(bs-41320P) per lane probed with TLR7 polyclonal antibody respectively, unconjugated (bsm-41320R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.


The THP-1 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-TLR7 antibody (bsm-41320R): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 μg/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.