bsm-41327M [Primary Antibody]
ZO-1 Monoclonal Antibody
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Host: Mouse

Target Protein: ZO-1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7082

Swiss Prot: Q07157

Source: Recombinant human ZO-1 protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ICC/IF(1:50-100)

Predicted Molecular Weight: 191


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with ZO-1 monoclonal antibody, unconjugated (bsm-41327M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.