bsm-41481M [Primary Antibody]
SARS-CoV-2 Nucleocapsid Protein (9D2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: SARS-CoV-2 Nucleocapsid protein

Immunogen Range: 1-419/419


Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 43740575

Swiss Prot: P0DTC9

Source: Recombinant SARS-CoV-2 Nucleocapsid Protein (full length)

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M PBS(pH7.4) with 0.03% Proclin300.

Storage: Store at -20°C for 12 months..

Background:

Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Size: 100ul

Concentration: ≥1 ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species: SARS-CoV-2

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SARS-CoV-2 N protein(WT) at 10ng; Lane 2: SARS-CoV-2 N protein(Q9H, P67S, P80R, P151L, S183Y) at 2ng; Lane 3: SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y,H145Y, R203K, G204R, T205I, S235F) at 2ng; Lane 4: SARS-CoV-2 N protein (Del204 & Del215) at 2ng; Lane 5: SARS-CoV-2 N protein (R203M & D377Y) at 2ng probed with anti-SARS-CoV-2 N protein monoclonal antibody, Unconjugated (bsm-41481M) at 1:1000 dilution and 4℃ overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60min at 37 ℃.


Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 NP (bs-41417P) at 2μg/mL (100μL/well) can bind anti-SARS-CoV-2 NP monoclonal antibody, the EC50 for this effect is 3.6-7.8ng/ml.