bsm-42057R [Primary Antibody]
AIF1 Recombinant Antibody
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Host: Rabbit

Target Protein: AIF1

Immunogen Range: 2-147/147


Clonality: Recombinant

Isotype: IgG

Entrez Gene: 11629

Swiss Prot: O70200

Source: Recombinant mouse AIF1 protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(WB(1:500-2000))

Predicted Molecular Weight: 16


Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with AIF1 monoclonal antibody, unconjugated (bsm-42057R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.