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Host: Mouse
Target Protein: MUC16
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 94025
Swiss Prot: Q8WXI7
Source: Recombinant human MUC16 Protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100uL
Concentration: 1mg/ml
Applications:
WB(WB(1:500-2000))
FCM(FCM(1μg/Test))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))
IF(ICC)(IF(ICC)(1:50-200))
Predicted Molecular Weight: 200
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian mucinous Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated(bsm-43121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial mucinous Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated(bsm-43121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated(bsm-43121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated(bsm-43121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with MUC16 monoclonal antibody, unconjugated (bsm-43121M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed OVCAR-3 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MUC16) monoclonal Antibody, unconjugated (bsm-43121M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The OVCAR-3 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-MUC16 antibody (bsm-43121M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 μg/test. Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.