bsm-4713M [Primary Antibody]
beta-Amyloid (1-42) Monoclonal Antibody
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Host: Mouse

Target Protein: beta-Amyloid 1-42

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 351

Source: KLH conjugated synthetic peptide derived from human beta-Amyloid (1-42)

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The cerebral and vascular plaques associated with Alzheimer's disease are mainly composed of Amyloid beta peptides. beta Amyloid is derived from cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of Amyloid precursor protein after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last Amyloid precursor protein processing step. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides are major constituents of the plaques and tangles that occur in Alzheimer's disease. beta Amyloid and peptides have been developed as tools for elucidating the biology of Alzheimer's disease.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(WB(1:500-2000))
IHC-P(IHC-P(1:500-5000))
IHC-F(IHC-F(1:500-5000))
IF(IHC-P)(IF(IHC-P)(1:200-2000))

Cross Reactive Species: Human
Rat
Bovine
Dog
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Chen, et al. "Downregulation of PI3K/Akt/mTOR signaling pathway in curcumin-induced autophagy in APP/PS1 double transgenic mice." European Journal of Pharmacology (2014).Read more>>
  • Shufang Na. et al. Chronic Neuroinflammation Induced by Lipopolysaccharide Injection into the Third Ventricle Induces Behavioral Changes. 2021 Jan 06Read more>>
  • Yunxiao Dou. et al. Presequence protease reverses mitochondria-specific amyloid--induced mitophagy to protect mitochondria. FASEB J. 2023 Apr;37(5):e2289Read more>>
VALIDATION IMAGES

100 ng Aβ1-42 Peptide (bs-0107P) per lane probed with beta-Amyloid(1-42) polyclonal antibody respectively, unconjugated (bsm-4713M) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.


Positive sample（left）：Alzheimer's Disease Brain Negative sample（right）：Normal Human Brain Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (beta-Amyloid(1-42)) Monoclona Antibody, Unconjugated (bsm-4713M) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0024) instructionsand DAB staining.