bsm-50185M [Primary Antibody]
p38 MAPK (6A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: p38 MAPK

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 1432

Swiss Prot: Q16539

Source: Synthetic Glutaldehyde peptide (KLH coupled) corresponding to C-terminal residues of human p38 MAP kinase

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation and other signals. p38 MAPK is relatively inactive in the non-phosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, _, _, _ and _, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor-_, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 and 6 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

Size: 100ul

Concentration: 4ug/ul

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1 : HeLa Cell Lysate; Lane 2 : 293T Cell Lysate; Lane 3 : K562 Cell Lysate; Lane 4 : NIH3T3 Cell Lysate; Lane 5 : PC12 Cell Lysate. WB analysis was performed using p38 MAPK (6A1) Monoclonal Antibody (bsm-50185M) at 4.0ug/mL (1:250) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at 37˚C.