DATASHEET
Host:
Mouse
Target Protein:
Complement C4 (gamma chain)
Clonality:
Monoclonal
Isotype:
IgG2b
Entrez Gene:
720
Swiss Prot:
P0C0L4
Source:
Protein purified from Human plasma
Purification:
Ammonium sulfate precipitation
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.
Background:
Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.