bsm-50249M [Primary Antibody]
IKK beta (42D1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: IKK beta

Clonality: Monoclonal

Isotype: IgG2b

Entrez Gene: 3551

Swiss Prot: O14920

Source: Recombinant human protein purified from E.coli (His-IKKbeta)

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 87


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1 : HeLa Cell Lysate; Lane 2 : 293T Cell Lysate; Lane 3 : C6 Cell Lysate; Lane 4 : NIH3T3 Cell Lysate; probed with IKK β (42D1) Monoclonal Antibody (bsm-50249M) at 1.0ug/mL (1:1000) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.


Lane 1 : IκB kinase alpha Recombinant Protein; Lane 2 : IκB kinase beta Recombinant Protein; Lane 3 : IκB kinase gamma Recombinant Protein; probed with IKK β (42D1) Monoclonal Antibody (bsm-50249M) at 1.0ug/mL (1:1000) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.