DATASHEET
Host:
Mouse
Target Protein:
MEK2
Clonality:
Monoclonal
Isotype:
IgG1
Entrez Gene:
5605
Swiss Prot:
P36507
Source:
Recombinant human protein purified from E.coli (His-MEK2)
Purification:
Ammonium sulfate precipitation
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.
Background:
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
VALIDATION IMAGES
Lane 1 : MEK1 Recombinant Protein; Lane 2 : MEK2 Recombinant Protein; Lane 3 : MEK3 Recombinant Protein; Lane 4 : MEK4 Recombinant Protein; Lane 5 : MEK5 Recombinant Protein; Lane 6 : MEK6 Recombinant Protein; Lane 7 : MEK7 Recombinant Protein; probed with MEK2 (1A1) Monoclonal Antibody (bsm-50250M) at 1.0ug/mL (1:1000) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.
Lane 1 : HeLa Cell Lysate; Lane 2 : U87mg Cell Lysate; Lane 3 : MCF7 Cell Lysate; Lane 4 : U937 Cell Lysate; probed with MEK2 (1A1) Monoclonal Antibody (bsm-50250M) at 2.0ug/mL (1:500) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.