DATASHEET
Host:
Mouse
Target Protein:
Complement C4 beta chain
Clonality:
Monoclonal
Isotype:
IgG1
Entrez Gene:
721
Swiss Prot:
P0C0L4
Source:
Protein purified from Human plasma
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.
Background:
Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Complement C4 β-chain (52H10) monoclonal Antibody, Unconjugated (bsm-50255M) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1: Human Serum (0.5uL); Probed with Complement C4 beta chain (52H10) Monoclonal Antibody, Unconjugated (bsm-50255M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Immunoprecipitation of Human Serum using Complement C4 beta chain (52H10) Monoclonal Antibody (bsm-50255M): Lane 1: Human Serum (1.0uL); Lane 2: Precipitated from Human Serum 1uL using 2ug of antibody; Lane 3: Precipitated from Human Serum 1uL using 5ug of antibody; Lane 4: Precipitated from PBS using 5ug of antibody.