Host: Mouse
Target Protein: Bid
Clonality: Monoclonal
Isotype: IgG2a
Entrez Gene: 637
Swiss Prot: P55957
Source: Recombinant human protein purified from E.coli(His-Bid)
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.
Background:
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 22
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Bid (8D2) monoclonal Antibody, Unconjugated (bsm-50264M) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1 : HeLa Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : K562 Cell Lysate ; Lane 4 : NCI-H460 Cell Lysate; probed with Bid (8D2) Monoclonal Antibody, unconjugated (bsm-50264M) at 1:20000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of A431 cell lysates using Bid (8D2) Monoclonal Antibody (bsm-50264M): Lane 1 : A431 Cell Lysate; Lane 2 : Precipitated from 200ug of Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50264M at 0.1ug/mL (1:10000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at Room Temperature.