bsm-50303M [Primary Antibody]
MEK1 (32G3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: MEK1

Clonality: Monoclonal

Isotype: IgG1 κ

Entrez Gene: 5604

Swiss Prot: Q02750

Source: Recombinant human protein purified from E.coli(His-MEK1)

Purification: Ammonium sulfate precipitation

Storage Buffer: HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Storage Condition: Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.

Background: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Size: 100ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1 : HeLa Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : PC12 Cell Lysate; Lane 4 : NIH3T3 Cell Lysate; probed with MEK1 (32G3) Monoclonal Antibody, unconjugated (bsm-50303M) at 1:5000 overnight at 4°C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.