bsm-50360M [Primary Antibody]
ILK (22F1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: ILK

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 3611

Swiss Prot: Q13418

Source: Recombinant human protein purified from E.coli (His-ILK)

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.

Background:

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 51


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1 : HeLa Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : PC12 Cell Lysate; Lane 4 : NIH3T3 Cell Lysate; probed with bsm-50360M ILK (AF22F1) Monoclonal Antibody at 1.0ug/mL (1:1000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at room temperature.