DATASHEET
Host:
Mouse
Target Protein:
p53
Clonality:
Monoclonal
Isotype:
IgG1
Entrez Gene:
7157
Swiss Prot:
P04637
Source:
Recombinant human protein purified from E.coli (His-p53)
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.
Background:
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
VALIDATION IMAGES
Lane 1 : 293T Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : Ramos Cell Lysate; probed with bsm-50362M p53 (2C1) Monoclonal Antibody at 0.5ug/mL (1:2000) and incubated at 4℃ overnight, followed secondary antibody incubation for 60min at room temperature.
Immunoprecipitation of p53 from 293T cells using p53 (2C1) Monoclonal Antibody (bsm-50362M). Lane 1 : 293T Cell Lysate; Lane 2 : Precipitated from 293T Cell Lysate (200ug) using 2ug of antibody; Lane 3 : Precipitated from 293T Cell Lysate (200ug) using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50362M at 0.5ug/mL (1:2000) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.