bsm-50375M [Primary Antibody]
Cyclin D1 (2G5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Cyclin D1

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 595

Swiss Prot: P24385

Source: Recombinant human protein purified from E.coli (His-Cyclin D1)

Purification: Ammonium sulfate precipitation

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.

Size: 100ul

Concentration: Not Determined

Predicted Molecular Weight: 34


Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang et al. Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice. (2015) World.J.Gastroenterol. 21:4195-209Read more>>
VALIDATION IMAGES

Lane 1 : MCF7 Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : C6 Cell Lysate; Lane 4 : L929 Cell Lysate; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 4.0ug/mL (1:250) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.


Lane 1 : Cyclin B1 Recombinant Protein; Lane 2 : Cyclin D1 Recombinant Protein; Lane 3 : Cyclin D2 Recombinant Protein; Lane 4 : Cyclin D3 Recombinant Protein; Lane 5 : Cyclin E1 Recombinant Protein; Lane 6 : Cyclin E2 Recombinant Protein; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 0.5ug/mL (1:2000) and incubated at 4℃ overnight, followed by secondary antibody incubation for 60min at room temperature.