bsm-50409M [Primary Antibody]
MRE11 (44B12) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: MRE11

Clonality: Monoclonal

Isotype: IgG2A/kappa

Entrez Gene: 4361

Swiss Prot: P49959

Source: Recombinant human protein purified from E.coli (GST-MRE11)

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 81


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1 : HeLa Cell Lysate; Lane 2 : SK-N-MC Cell Lysate; Lane 3 : K562 Cell Lysate; Lane 4 : Ramos Cell Lysate; probed bsm-50409M MRE11 (44B12) Monoclonal Antibody with 0.2ug/mL (1:5000) dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.