bsm-51011M [Primary Antibody]
beta actin (8H10D10) Monoclonal Antibody
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support@biossusa.com
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Mouse

Target Protein: beta actin

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 60

Swiss Prot: P60709

Source: ACTB recombinant protein is used to produce this monoclonal antibody.

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:1000-5000)
IF(1:200-1000)
IHC-F(1:200-1000)
IHC-P(1:200-1000)

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTB | beta-Actin Monoclonal Antibody, Unconjugated (bsm-51011M) at 1:200 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.