DATASHEET
Host:
Mouse
Target Protein:
Lactoferrin
Clonality:
Monoclonal
Isotype:
IgG1
Entrez Gene:
4057
Swiss Prot:
P02788
Source:
This LTF Monoclonal antibody is generated from mouse immunized with LTF recombinant protein.
Purification:
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis.
VALIDATION IMAGES
25 ug total protein per lane of various lysates (see on figure) probed with Lactoferrin monoclonal antibody, unconjugated (bsm-51024M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Lactoferrin Monoclonal Antibody, Unconjugated (bsm-51024M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Lactoferrin Monoclonal Antibody, Unconjugated (bsm-51024M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.