Host: Mouse
Target Protein: MAP4K3
Clonality: Monoclonal
Isotype: IgM
Entrez Gene: 8491
Swiss Prot: Q8IVH8
Source: This MAP4K3 monoclonal antibody is generated from mouse immunized with MAP4K3 recombinant protein.
Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 0.5ug/ul
Predicted Molecular Weight: 101
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: HepG2 Cell lysates; Lane 2: MCF-7 Cell lysates; probed with MAP4K3 (219CT8.3.1) Monoclonal Antibody, unconjugated (bsm-51067M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MAP4K3 (219CT8.3.1) Monoclonal Antibody (bsm-51067M) at 1:100 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.