bsm-51106M [Primary Antibody]
Human Ig Kappa light chain Monoclonal Antibody
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Host: Mouse

Target Protein: Human Ig Kappa light chain

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 3514

Swiss Prot: P01834

Source: Recombinant human Kappa light chain

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two types of light chains designated as kappa and lambda (1). Light chain types are based on differences in the amino acid sequence in the constant region of the light chain. If a cell is unsuccessful in rearranging both of its kappa light chain genes, it then attempts to make a lambda light chain. If a cell successfully rearranges a lambda light chain gene, it will be a B cell that makes an immunoglobulin with a lambda light chain (2).

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:1000-5000)
ELISA(1:5000-10000)

Predicted Molecular Weight: 11 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human Serum 8μl Lysates. Probed with Human Ig Kappa light chain monoclonal Antibody, unconjugated (bsm-51106M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.