bsm-51116M [Primary Antibody]
LGR5/GPR49 (9C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: LGR5/GPR49

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 8549

Swiss Prot: O75473

Source: This LGR5/GPR49 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 689-719 amino acids from human LGR5/GPR49.

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 100


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: K562 Cell lysates; probed with LGR5/GPR49 (349CT16.1.3.1) Monoclonal Antibody, unconjugated (bsm-51116M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.