bsm-51186M [Primary Antibody]
IL1B | IL-1 beta Monoclonal Antibody
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Host: Mouse

Target Protein: IL1B | IL-1 beta

Clonality: Monoclonal

Isotype: IgG1, k

Entrez Gene: 3553

Swiss Prot: P01584

Source: Recombinant human IL-1 beta protein

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:200-2000)

Predicted Molecular Weight: 32 kDa


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

RAW 264.7 (M) cells were treated with or without LPS (100ng/ml) for 6 h, A added (300ng/ml) for 3 h, 25 μg total protein per lane of cell lysates (see on figure) probed with IL1B | IL-1 beta monoclonal antibody, unconjugated (bsm-51186M) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


THP-1 (H) cells were treated with or without TPA (80nM) for 24 h, LPS (100ng/ml) for 6 h, Brefeldin A (300ng/ml) for 3 h, 25 μg total protein per lane of cell lysates (see on figure) probed with IL1B | IL-1 beta monoclonal antibody, unconjugated (bsm-51186M) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.