bsm-51201M [Primary Antibody]
PODXL (2C4) Monoclonal Antibody
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Host: Mouse

Target Protein: PODXL

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 5420

Swiss Prot: O00592

Source: This PODXL antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the C-terminal region of human PODXL.

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

Size: 100µL

Concentration: 0.5ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF

Predicted Molecular Weight: 59


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

A549 cell lysates probed with PODXL (632CT5.11.4) Monoclonal Antibody, unconjugated (bsm-51201M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.