DATASHEET
Host:
Mouse
Target Protein:
PODXL
Clonality:
Monoclonal
Isotype:
IgG1
Entrez Gene:
5420
Swiss Prot:
O00592
Source:
This PODXL antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the C-terminal region of human PODXL.
Purification:
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.