bsm-51215M [Primary Antibody]
Hsp90 (3C9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Hsp90

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 3320

Swiss Prot: P07900

Source: This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 150-400 amino acids from human.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 85


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gaoqian Zhao. et al. Biomimetic Platform Based on Mesoporous Platinum for Multisynergistic Cancer Therapy. Acs Biomater Sci Eng. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Ali Tugrul Akin. et al. Chloroquine attenuates chronic hypoxia-induced testicular damage via suppressing endoplasmic reticulum stress and apoptosis in experimental rat model. CLIN EXP PHARMACOL P. 2022 May 17Read more>>
  • Kerschbamer Emanuela. et al. CHD8 suppression impacts on histone H3 lysine 36 trimethylation and alters RNA alternative splicing. NUCLEIC ACIDS RES. 2022 Dec;50(22):12809-12828Read more>>
  • Osman Ahmet Polat. et al. Evaluation of histologic, antiapoptotic and antioxidant effects of melatonin against the acute ocular toxicity of Cisplatin. TISSUE CELL. 2023 Dec;85:102226Read more>>
  • G.F. Sengl. et al. Hsc70 phosphorylation patterns and calmodulin regulate AP2 Clathrin-Coated-Vesicle life span for cell adhesion protein transport. BBA-MOL CELL RES. 2024 Jan;1871:119611Read more>>
VALIDATION IMAGES

Lane 1: L6; Lane 2: NIH/3T3; Lane 3: T47D; Lane 4: A2058; Lane 5: HepG2; Lane 6: K562; Lane 7: HeLa Cell lysates; probed with Hsp90 (3C9) Monoclonal Antibody, unconjugated (bsm-51215M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Rat Placenta tissue lysates ; Lane 6: Human U251 cell lysates ; Lane 7: Human Molt-4 cell lysates ; Lane 8: Human HL60 cell lysates ; Lane 9: Human A549 cell lysates probed with HSP90 Monoclonal Antibody, Unconjugated (bsm-51215M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.