bsm-51226M [Primary Antibody]
MBD3 (5C3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: MBD3

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 53615

Swiss Prot: O95983

Source: Purified His-tagged MBD3 protein was used to produced this monoclonal antibody.

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 33


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: K562 Cell lysates; probed with MBD3 (5C3) Monoclonal Antibody, unconjugated (bsm-51226M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.