bsm-51236M [Primary Antibody]
GOLPH3 (9A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: GOLPH3

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 64083

Swiss Prot: Q9H4A6

Source: Purified His-tagged GOLPH3 protein was used to produced this monoclonal antibody.

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 34


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: 293 lysates; Lane 2: MDA-MB-231 Cell lysates; probed with GOLPH3 (9A1) Monoclonal Antibody, unconjugated (bsm-51236M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.