bsm-51242M [Primary Antibody]
VRK1 (5C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: VRK1

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 7443

Swiss Prot: Q99986

Source: Purified His-tagged VRK1 protein was used to produced this monoclonal antibody.

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 45


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HeLa Cell lysates; Lane 2: HL-60Cell lysates; probed with VRK1 (1015CT2.1.1) Monoclonal Antibody, unconjugated (bsm-51242M) at 1:5000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.