bsm-51259M [Primary Antibody]
USP25 (7C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: USP25

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 29761

Swiss Prot: Q9UHP3

Source: This USP25 antibody is generated from a mouse immunized with a recombinant protein from human USP25.

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 122


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Daudi Cell lysates; probed with USP25 (7C1) Monoclonal Antibody, unconjugated (bsm-51259M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.