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Host: Mouse
Target Protein: PPT1
Clonality: Monoclonal
Isotype: IgG1
Entrez Gene: 5538
Swiss Prot: P50897
Source: This PPT1 antibody is generated from a mouse immunized with a recombinant protein from human PPT1.
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: 0.5ug/ul
Applications:
WB(1:300-5000)
ELISA(1:50-200)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF
Predicted Molecular Weight: 34
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
HeLa Cell lysates; probed with PPT1 (10G4) Monoclonal Antibody, unconjugated (bsm-51262M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
HepG2 Cell lysates; probed with PPT1 (10G4) Monoclonal Antibody, unconjugated (bsm-51262M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Human cerebellum tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PPT1 (10G4) Monoclonal Antibody (bsm-51262M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse cerebellum tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with PPT1 (10G4) Monoclonal Antibody, Unconjugated (bsm-51262M) at 1:25 for 1hr at 37°C, followed by a conjugated secondary antibody and DAB staining.