bsm-51274M [Primary Antibody]
MAFK (8C5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: MAFK

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 7975

Swiss Prot: O60675

Source: This MAFK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human MAFK.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 18


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: MAFK Cell lysates; probed with MAFK (8C5) Monoclonal Antibody, unconjugated (bsm-51274M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Hela cells probed with using MAFK (8C5) Monoclonal Antibody (green) unconjugated bsm-51274M at 1:25 for 30 minutes followed by A488 secondary antibody at 1:400 compared to an isotype control of mouse IgG1(blue).