bsm-51278M [Primary Antibody]
JUN (6C7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: JUN

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 3725

Swiss Prot: P05412

Source: This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: 293 Cell lysates; Lane 2: NIH/3T3 Cell lysates; probed with JUN (6C7) Monoclonal Antibody, unconjugated (bsm-51278M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.