DATASHEET
Host:
Mouse
Target Protein:
IF4E
Clonality:
Monoclonal
Isotype:
IgG1
Entrez Gene:
1977
Swiss Prot:
P06730
Source:
This IF4E antibody is generated from a mouse immunized with a recombinant protein of human IF4E.
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
VALIDATION IMAGES
Lane 1: HeLa Cell lysates; Lane 2: MCF-7 Cell lysates; Lane 3: K562 Cell lysates; Lane 4: NIH/3T3 Cell lysates; Lane 5: PC-12 Cell lysates; probed with IF4E (4C5) Monoclonal Antibody, unconjugated (bsm-51305M) at 1:4000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 cells labeled with IF4E (4C5) Monoclonal Antibody (bsm-51305M) at 1/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).