Host: Mouse
Target Protein: BID
Clonality: Monoclonal
Isotype: IgG1
Entrez Gene: 637
Swiss Prot: P55957
Source: This BID antibody is generated from a mouse immunized with a recombinant protein of human BID.
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 0.5ug/ul
Predicted Molecular Weight: 22
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Jurkat Cell lysates; Lane 2: A431 Cell lysates; Lane 3: THP-1 Cell lysates; Lane 4: 293T/17 Cell lysates; probed with BID (9C7) Monoclonal Antibody, unconjugated (bsm-51306M) at 1:x000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeled with BID (9C7) Monoclonal Antibody (bsm-51306M) at 1/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded human bladder tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BID (9C7) Monoclonal Antibody (bsm-51306M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.