bsm-51308M [Primary Antibody]
KRT19 (9C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: KRT19

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 3880

Swiss Prot: P08727

Source: This antibody is generated from a mouse immunized with a recombinant protein of human.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 44


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeled with KRT19 (979CT14.3.1) Monoclonal Antibody (bsm-51308M) at 1/25 dilution, followed by secondary antibody (green) and nuclear counter stain with DAPI (blue).


Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with KRT19 (979CT14.3.1) Monoclonal Antibody (bsm-51308M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.