bsm-51311M [Primary Antibody]
RAB3A (5B7) Monoclonal Antibody
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Host: Mouse

Target Protein: RAB3A

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 5864

Swiss Prot: P20336

Source: This RAB3A antibody is generated from a mouse immunized with a recombinant protein.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.

Size: 100µL

Concentration: 0.5ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(ICC)(1:200-400)
IF(1:50-200)

Predicted Molecular Weight: 25


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: mouse brain lysates; Lane 2: rat brain lysates; probed with RAB3A (5B7) Monoclonal Antibody, unconjugated (bsm-51311M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labeled with RAB3A (5B7) Monoclonal Antibody (bsm-51311M) at 1/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).


human brain lysates probed with RAB3A (5B7) Polyclonal Antibody, unconjugated (bsm-51311M) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


PC-12 cells probed with using RAB3A (5B7) Monoclonal Antibody, bsm-51311M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG (blue).


Lane 1: Cerebrum lysates probed with RAB3A (5B7) Monoclonal Antibody, Unconjugated (bsm-51311M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates probed with RAB3A (5B7) Monoclonal Antibody, Unconjugated (bsm-51311M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.