bsm-51318M [Primary Antibody]
DENR (2C9) Monoclonal Antibody
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Host: Mouse

Target Protein: DENR

Clonality: Monoclonal

Isotype: IgG2b

Entrez Gene: 8562

Swiss Prot: O43583

Source: This DENR antibody is generated from a mouse immunized with a recombinant protein of human DENR.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

May be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. Involved in translation initiation; promotes recruitmnet of aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1.

Size: 100µL

Concentration: 0.5ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
IF(1:50-200)

Predicted Molecular Weight: 22


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with DENR (2C9) Monoclonal Antibody (bsm-51318M) at 1/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).


Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with DENR (2C9) Monoclonal Antibody (bsm-51318M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.


Lane 1: A431; Lane 2: human skeletal muscle lysates; Lane 3: human brain Cell lysates; probed with DENR (2C9) Polyclonal Antibody, unconjugated (bsm-51318M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Hela cells probed with using DENR (2C9) Monoclonal Antibody bsm-51318M at 1:25 for 60 minutes at 37ºC followed by secondary antibody at 1:400 (green), compared to an isotype control of mouse IgG2b (blue).