bsm-51334M [Primary Antibody]
RAB27A (9C6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: RAB27A

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 5873

Swiss Prot: P51159

Source: This RAB27A antibody is generated from a mouse immunized with a recombinant protein of human RAB27A.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 25


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liang Min. et al. Astragaloside IV Suppresses the Effects of Hepatocellular Carcinoma Cells on Proliferation, Angiogenesis, and Invasion in Human Umbilical Vein Endothelial Cells by Controlling Exosomes by Inhibiting Rab27a. J FOOD BIOCHEM. 2023;2023:8812742Read more>>
VALIDATION IMAGES

Lane 1: K562 Cell lysates; probed with RAB27A (9C6) Monoclonal Antibody, unconjugated (bsm-51334M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


K562 cells probed with using RAB27A (9C6) Monoclonal Antibody bsm-51334M at 1:25 for 60 minutes followed by A488 secondary antibody at 1:200 (green) compared to an isotype control of mouse IgG1 (blue).


Lane 1: Human K562 cell lysates; Lane 2: Human Jurkat cell lysates probed with RAB27A Monoclonal Antibody, Unconjugated (bsm-51334M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.