bsm-51337M [Primary Antibody]
MCM3 (3C3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: MCM3

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 4172

Swiss Prot: P25205

Source: This MCM3 antibody is generated from a mouse immunized with a recombinant protein of human MCM3.

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Size: 100ul

Concentration: 0.5ug/ul

Predicted Molecular Weight: 91


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: CHO; Lane 2: NIH/3T3; Lane 3: Hela; Lane 4: HepG2; Lane 5: C2C12; Lane 6: PC-12 cell lysates; probed with MCM3 (3C3) Polyclonal Antibody, unconjugated (bsm-51337M) at 1:4000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.